Multi-color fluorescent DNA analysis in an optofluidic chip

In a commercial microfluidic chip (LioniX BV), a plug of DNA molecules with a volume of ~605 pl was injected and the DNA molecules were CE-separated with a high relative sizing accuracy of > 99% [1]. Through an optical waveguide inscribed by femtosecond-laser writing [2] a laser was launched perpendicularly into the microfluidic channel. A photomultiplier collected the fluorescence signals from a small detection window with a limit of detection of ~8 DNA molecules [3].